Improving Environmental DNA Sensitivity for Dreissenid Mussels
June 29, 2022
Enter the password to open this PDF file:
File name:
-
File size:
-
Title:
-
Author:
-
Subject:
-
Keywords:
-
Creation Date:
-
Modification Date:
-
Creator:
-
PDF Producer:
-
PDF Version:
-
Page Count:
-
Page Size:
-
Fast Web View:
-
Preparing document for printing…
0%
of
Share
{
"description": "",
"ideasArticleShareLabel": "Share",
"networks": [
"linkedin",
"twitter",
"email"
]
}
Stantec’s Dr. Nathaniel Marshall and associates discuss improving eDNA sensitivity for invasive mussels in the scientific journal, Water
{
"text": "
The Cooperative Institute for Great Lakes Research (CIGLR) and the National Oceanic and Atmospheric Administration’s Great Lakes Environmental Research Laboratory collaborated with Dr. Nathaniel Marshall to improve eDNA sensitivity for Dreissenid Mussels. The recent genetic revolution through the analysis of aquatic environmental DNA (eDNA) has become a powerful tool for improving the detection of rare and/or invasive species. For the majority of eDNA studies, genetic assays are designed to target mitochondrial genes commonly referred to as “barcode” regions. However, unlike the typical structure of an animal mitochondrial genome, those for the invasive zebra and quagga mussels are greatly expanded with large extended tandem repeat regions. These sections of repeated DNA can appear hundreds of times within the genome compared to a single copy for the mitochondrial barcode genes. This higher number of target copies per mitochondrial genome presents an opportunity to increase eDNA assay sensitivity for these species. Therefore, we designed and evaluated new eDNA assays to target the extended repeat sections for both zebra and quagga mussels.
These assays lower the limit of detection of genomic DNA by 100-fold for zebra mussels and 10-fold for quagga mussels. Additionally, these newly developed assays provided longer durations of detection during degradation mesocosm experiments and greater sensitivity for eDNA detection from water samples collected across western Lake Erie compared to standard assays targeting mitochondrial genes. This work illustrates how understanding the complete genomic structure of an organism can improve eDNA analysis.
" }Share
{
"description": "",
"ideasArticleShareLabel": "Share",
"networks": [
"linkedin",
"twitter",
"email"
]
}
-
Focused on the development and implementation of environmental DNA (eDNA), Nathaniel has worked on freshwater mussel conservation and the early detection of invasive species.
Contact Nathaniel
{
"headline": "Related Ideas",
"link": {
"text":"View More Ideas",
"url":"\/en\/ideas\/market\/environment.html"
},
"newTab": false,
"uuid": "9a63fbb3\u002Da3ed\u002D4081\u002Dade8\u002D7b99acae1053",
"themeClass": "home-related-items-2",
"tabs": [
{
"headline": "",
"active": true,
"tabLabel": "Slide Card",
"pages": [
{
"pageExists": true,
"cardLabel": "Slide Card 0 - Managing your nature-related risks and opportunities for enhanced long-term sustainability",
"link": {
"text":"Managing your nature\u002Drelated risks and opportunities for enhanced long\u002Dterm sustainability",
"url":"\/en\/ideas\/content\/video\/2025\/managing\u002Dyour\u002Dnature\u002Drelated\u002Drisks\u002Dopportunities\u002Dfor\u002Denhanced\u002Dlong\u002Dterm\u002Dsustainability.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/video\/0004\/nature\u002Drelated\u002Drisks\u002Desg.jpg",
"overwriteHeadline": "Managing your nature\u002Drelated risks and opportunities for enhanced long\u002Dterm sustainability",
"overwriteSubhead": "Video",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 1 - Nature-based solutions: Using engineering and nature to solve environmental challenges",
"link": {
"text":"Nature\u002Dbased solutions: Using engineering and nature to solve environmental challenges",
"url":"\/en\/ideas\/content\/video\/2024\/nbs\u002Dusing\u002Dengineering\u002Dnature\u002Dto\u002Dsolve\u002Denvironmental\u002Dchallenges.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/video\/0004\/nbs\u002Dusing\u002Dengineering\u002Dnature.jpg",
"overwriteHeadline": "Nature\u002Dbased solutions: Using engineering and nature to solve environmental challenges",
"overwriteSubhead": "Video",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 2 - Stantec’s Top 10 Ideas from 2023",
"link": {
"text":"Stantec’s Top 10 Ideas from 2023",
"url":"\/en\/ideas\/content\/blog\/2023\/stantecs\u002Dtop\u002D10\u002Dideas\u002Dfrom\u002D2023.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/024\/seahorse\u002Dhotels\u002D1.jpg",
"overwriteHeadline": "Stantec’s Top 10 Ideas from 2023",
"overwriteSubhead": "Blog Post",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 3 - Bats in buildings: How airborne eDNA can help you identify bat species at risk",
"link": {
"text":"Bats in buildings: How airborne eDNA can help you identify bat species at risk",
"url":"\/en\/ideas\/content\/blog\/2023\/bats\u002Dbuildings\u002Dhow\u002Dairborne\u002Dedna\u002Dcan\u002Dhelp\u002Dyou\u002Didentify\u002Dbat\u002Dspecies\u002Dat\u002Drisk.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/023\/brown\u002Dbat\u002Dadobestock\u002D547238326.jpg",
"overwriteHeadline": "Bats in buildings: How airborne eDNA can help you identify bat species at risk",
"overwriteSubhead": "Blog Post",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 4 - Real-world Examples of Biological Monitoring with Environmental DNA (eDNA)",
"link": {
"text":"Real\u002Dworld Examples of Biological Monitoring with Environmental DNA (eDNA)",
"url":"\/en\/ideas\/content\/technical\/2023\/real\u002Dworld\u002Dexamples\u002Dbiological\u002Dmonitoring\u002Dwith\u002Dedna.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/pdf\u002Dpromo\u002Dimages\/003\/transmission\u002Dline\u002Dtower\u002Dadobestock\u002D534987828.jpg",
"overwriteHeadline": "Real\u002Dworld Examples of Biological Monitoring with Environmental DNA (eDNA)",
"overwriteSubhead": "Report",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 5 - When freshwater mussel surveys are needed, eDNA can show the way",
"link": {
"text":"When freshwater mussel surveys are needed, eDNA can show the way",
"url":"\/en\/ideas\/content\/blog\/2022\/when\u002Dfreshwater\u002Dmussel\u002Dsurveys\u002Dare\u002Dneeded\u002Dedna\u002Dcan\u002Dshow\u002Dthe\u002Dway.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/022\/freshwater\u002Dmussel\u002Dsurveys\u002D2.jpg",
"overwriteHeadline": "When freshwater mussel surveys are needed, eDNA can show the way",
"overwriteSubhead": "Blog Post",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 6 - Improving Environmental DNA Sensitivity for Dreissenid Mussels",
"link": {
"text":"Improving Environmental DNA Sensitivity for Dreissenid Mussels",
"url":"\/en\/ideas\/content\/technical\/2022\/improving\u002Denvironmental\u002Ddna\u002Dsensitivity\u002Dfor\u002Ddreissenid\u002Dmussels.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/pdf\u002Dpromo\u002Dimages\/003\/lake\u002Derie\u002Dadobestock\u002D288903602.jpg",
"overwriteHeadline": "Improving Environmental DNA Sensitivity for Dreissenid Mussels",
"overwriteSubhead": "Technical Paper",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": false,
"cardLabel": "Slide Card 7 - ",
"link": {
"text":"",
"url":""
},
"promoImage": "",
"overwriteHeadline": "",
"overwriteSubhead": "",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 8 - Evaluating environmental DNA metabarcoding as a survey tool for unionid mussel assessments",
"link": {
"text":"Evaluating environmental DNA metabarcoding as a survey tool for unionid mussel assessments",
"url":"\/en\/ideas\/content\/technical\/2022\/evaluating\u002Denvironmental\u002Ddna\u002Dmetabarcoding\u002Dsurvey\u002Dtool\u002Dunionid\u002Dmussel\u002Dassessments.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/022\/freshwater\u002Dmussels\u002Dadobestock\u002D304732119.jpg",
"overwriteHeadline": "Evaluating environmental DNA metabarcoding as a survey tool for unionid mussel assessments",
"overwriteSubhead": "Technical Paper",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": false,
"cardLabel": "Slide Card 9 - ",
"link": {
"text":"",
"url":""
},
"promoImage": "",
"overwriteHeadline": "",
"overwriteSubhead": "",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 10 - Using Environmental DNA (eDNA) to Improve Biological Assessments",
"link": {
"text":"Using Environmental DNA (eDNA) to Improve Biological Assessments",
"url":"\/en\/ideas\/content\/article\/2022\/using\u002Denvironmental\u002Ddna\u002Dedna\u002Dto\u002Dimprove\u002Dbiological\u002Dassessments.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/pdf\u002Dpromo\u002Dimages\/003\/trout\u002Dadobestock\u002D493802908.jpg",
"overwriteHeadline": "Using Environmental DNA (eDNA) to Improve Biological Assessments",
"overwriteSubhead": "Published Article",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 11 - Global Leaders in Applied eDNA",
"link": {
"text":"Global Leaders in Applied eDNA",
"url":"\/en\/ideas\/content\/video\/2021\/global\u002Dleaders\u002Din\u002Dapplied\u002Dedna.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/video\/0002\/stantec\u002Dedna\u002Dstock\u002D176201.jpg",
"overwriteHeadline": "Global Leaders in Applied eDNA",
"overwriteSubhead": "Video",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 12 - Bats from scats: Using eDNA to safely identify protected bat species in a time of COVID-19",
"link": {
"text":"Bats from scats: Using eDNA to safely identify protected bat species in a time of COVID\u002D19",
"url":"\/en\/ideas\/content\/blog\/2020\/bats\u002Dfrom\u002Dscats\u002Dusing\u002Dedna\u002Dto\u002Dsafely\u002Didentify\u002Dprotected\u002Dbat\u002Dspecies\u002Din\u002Da\u002Dtime\u002Dof\u002Dcovid\u002D19.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/013\/bat\u002Deptesicus\u002Dfuscus.jpg",
"overwriteHeadline": "Bats from scats: Using eDNA to safely identify protected bat species in a time of COVID\u002D19",
"overwriteSubhead": "Blog Post",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 13 - Stantec ERA Issue 02 | The Digital Issue",
"link": {
"text":"Stantec ERA Issue 02 | The Digital Issue",
"url":"\/en\/ideas\/content\/publication\/2019\/stantec\u002Dera\u002Dissue\u002D02\u002Dthe\u002Ddigital\u002Dissue.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/pdf\u002Dpromo\u002Dimages\/001\/era\u002Dissue\u002D02\u002D1.jpg",
"overwriteHeadline": "Stantec ERA Issue 02 | The Digital Issue",
"overwriteSubhead": "Publication",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 14 - Embracing a new eDNA technology",
"link": {
"text":"Embracing a new eDNA technology",
"url":"\/en\/ideas\/content\/blog\/2018\/embracing\u002Da\u002Dnew\u002Dedna\u002Dtechnology.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/ideas\/blogs\/010\/embracing\u002Dnew\u002Dedna\u002Dtechnology\u002D2.jpg",
"overwriteHeadline": "Embracing a new eDNA technology",
"overwriteSubhead": "Blog Post",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": true,
"cardLabel": "Slide Card 15 - 18 environmental DNA applications to help achieve nature positive goals",
"link": {
"text":"18 environmental DNA applications to help achieve nature positive goals",
"url":"\/en\/services\/digital\/edna\/what\u002Dis\u002Denvironmental\u002Ddna.html"
},
"promoImage": "\/content\/dam\/stantec\/images\/homepage\u002Dhero\/2023\/applying\u002DeDNA\u002Dhero\u002D2800x1640.jpg",
"overwriteHeadline": "18 environmental DNA applications to help achieve nature positive goals",
"overwriteSubhead": "Interactive",
"aorLabel": "Architect",
"architectOfRecord": ""
},
{
"pageExists": false,
"cardLabel": "Slide Card 16 - ",
"link": {
"text":"",
"url":""
},
"promoImage": "",
"overwriteHeadline": "",
"overwriteSubhead": "",
"aorLabel": "Architect",
"architectOfRecord": ""
}
]
},
{
"headline": "",
"active": false,
"tabLabel": "Slide Card",
"pages": [
]
},
{
"headline": "",
"active": false,
"tabLabel": "Slide Card",
"pages": [
]
}
]
}
{
"column1": {
headerLink: {
"text": "expertise",
"url": "\/en\/markets.html",
"target": ""
},
links: [
{
"text": "Buildings",
"url": "\/en\/markets\/buildings.html",
"target": "",
},
{
"text": "Community Development",
"url": "\/en\/services\/community\u002Ddevelopment.html",
"target": "",
},
{
"text": "Energy",
"url": "\/en\/markets\/energy.html",
"target": "",
},
{
"text": "Environment",
"url": "\/en\/markets\/environment.html",
"target": "",
},
{
"text": "Federal Government",
"url": "\/en\/markets\/federal\u002Dgovernment.html",
"target": "",
},
{
"text": "Mining, Minerals \x26 Metals",
"url": "\/en\/markets\/mining.html",
"target": "",
},
{
"text": "Transportation",
"url": "\/en\/markets\/transportation.html",
"target": "",
},
{
"text": "Water",
"url": "\/en\/markets\/water.html",
"target": "",
},
{
"text": "View All Markets",
"url": "\/en\/markets.html",
"target": "",
},
{
"text": "View All Services",
"url": "\/en\/services.html",
"target": "",
}
]
},
"column2": {
headerLink: {
"text": "initiatives",
"url": "\/en\/markets.html",
"target": ""
},
links: [
{
"text": "Climate Solutions",
"url": "\/en\/markets\/climate\u002Dsolutions.html",
"target": "",
},
{
"text": "Digital",
"url": "\/en\/services\/digital.html",
"target": "",
},
{
"text": "Coastal Resilience",
"url": "\/en\/markets\/coastal\u002Dmarine.html",
"target": "",
},
{
"text": "Ecosystem Restoration",
"url": "\/en\/services\/ecosystem\u002Drestoration.html",
"target": "",
},
{
"text": "Energy Transition",
"url": "\/en\/markets\/energy\u002Dtransition.html",
"target": "",
},
{
"text": "Smart Cities",
"url": "\/en\/markets\/smart\u002Dcities.html",
"target": "",
},
{
"text": "Sustainability",
"url": "\/en\/services\/sustainability.html",
"target": "",
},
{
"text": "International Institutions",
"url": "\/en\/services\/international\u002Dinstitutions.html",
"target": "",
}
]
},
"column3a": {
headerLink: {
"text": "ideas",
"url": "\/en\/ideas.html",
"target": ""
},
links: [
{
"text": "Visit Our Hub",
"url": "\/en\/ideas.html",
"target": "",
}
]
},
"column3b": {
headerLink: {
"text": "about",
"url": "\/en\/about.html",
"target": ""
},
links: [
{
"text": "Company Overview",
"url": "\/en\/about.html",
"target": "",
},
{
"text": "Leadership",
"url": "\/en\/about\/corporate\u002Dleadership.html",
"target": "",
},
{
"text": "Board of Directors",
"url": "\/en\/about\/corporate\u002Dgovernance\/board\u002Dof\u002Ddirectors.html",
"target": "",
},
{
"text": "Corporate Governance",
"url": "\/en\/about\/corporate\u002Dgovernance.html",
"target": "",
},
{
"text": "Corporate Sustainability",
"url": "\/en\/about\/sustainability.html",
"target": "",
},
{
"text": "Indigenous Relations",
"url": "\/en\/about\/indigenous\u002Drelations.html",
"target": "",
},
{
"text": "Community Engagement",
"url": "\/en\/about\/community\u002Dengagement.html",
"target": "",
},
{
"text": "Contact Us",
"url": "\/en\/about\/contact\u002Dus.html",
"target": "",
},
{
"text": "News",
"url": "\/en\/news\u002Dmedia.html",
"target": "",
},
{
"text": "Video",
"url": "\/en\/ideas\/watch.html",
"target": "",
},
{
"text": "Find An Office",
"url": "\/en\/offices\/office\u002Dfinder.html",
"target": "",
},
{
"text": "Supplier Information",
"url": "\/en\/about\/supplier\u002Dinformation.html",
"target": "",
}
]
},
"column4a": {
headerLink: {
"text": "careers",
"url": "\/en\/careers.html",
"target": ""
},
links: [
{
"text": "Our Better Together Culture",
"url": "\/en\/ideas\/careers.html",
"target": "",
},
{
"text": "Veteran \x26 Military",
"url": "\/en\/careers\/veteran\u002Dmilitary.html",
"target": "",
},
{
"text": "Students \x26 Graduates",
"url": "\/en\/careers\/students\u002Dgraduates.html",
"target": "",
},
{
"text": "Search \x26 Apply",
"url": "https:\/\/stantec.jobs\/",
"target": "_blank",
}
]
},
"column4b": {
headerLink: {
"text": "investors",
"url": "https:\/\/investors.stantec.com",
"target": "_blank"
},
links: [
]
},
"socialMediaTitle": "follow us",
"socialMediaLinks": [
{
"url": "https:\/\/www.facebook.com\/StantecInc",
"imagePath": "\/content\/dam\/stantec\/images\/Icons\/icon\u002Dfacebook.svg",
"imageAltText": "Facebook",
},
{
"url": "https:\/\/twitter.com\/Stantec",
"imagePath": "\/content\/dam\/stantec\/images\/Icons\/icon\u002Dtwitter.svg",
"imageAltText": "Twitter",
},
{
"url": "https:\/\/www.instagram.com\/stantec\/",
"imagePath": "\/content\/dam\/stantec\/images\/Icons\/instagram.svg",
"imageAltText": "Instagram",
},
{
"url": "https:\/\/www.linkedin.com\/company\/stantec\/",
"imagePath": "\/content\/dam\/stantec\/images\/Icons\/icon\u002DlinkedIn.svg",
"imageAltText": "LinkedIn",
},
{
"url": "https:\/\/www.youtube.com\/user\/StantecInc",
"imagePath": "\/content\/dam\/stantec\/images\/Icons\/icon\u002Dyoutube.svg",
"imageAltText": "YouTube",
}
],
"logoImage": "\/content\/dam\/stantec\/images\/Icons\/logo\u002Dlight.svg",
"logoAltText": "Stantec",
"phoneLabel": "Phone: ",
"phone": "1\u002D866\u002D782\u002D6832",
"supportEmailLabel": "Media Requests:",
"supportEmail": "media@stantec.com",
"mediaEmailLabel": " ",
"mediaEmail": " ",
"copyrightText": "® 2026 Stantec, All rights reserved.",
"copyrightLinks": [
{
"text": "Copyright \x26 Privacy Policy",
"url": "\/en\/copyright.html",
"target": ""
},
{
"text": "Portals",
"url": "\/en\/portals.html",
"target": ""
},
{
"text": "Site Map",
"url": "\/en\/sitemap.html",
"target": ""
}
]
}