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Laser study on 170-yr-old thumbnails re-writes history of Franklin

Low lead levels and zinc deficiency found in crewmember John Hartnell


A study conducted by a group of Canadian scientists published in the Journal of Archaeological Science: Reports debunks continued myths surrounding the demise of the Franklin Expedition. Thumbnail samples of John Hartnell, crew member of the HMS Terror, found that the long believed cause of death due to lead poisoning is inaccurate.

Led by Dr. Jennie Christensen Founder of TrichAnalytics, and in partnership with the University of Saskatchewan, University of Victoria, University of Ottawa, and Canadian Light Source (CLS), scientists used synchrotron micro-XRF mapping, laser ablation technology, and stable isotope analysis to examine metal exposure and diet throughout the early expedition. The study found that John Hartnell was chronically zinc-deficient, which possibly led to immuno-suppression and ultimately, tuberculosis and death. Zinc plays an integral role in vitamin A metabolism, and deficiencies in zinc and vitamin A can result in compromised immune function and diminished ability to fight infections, including tuberculosis. As malnourishment and zinc deficiency can have behavioral symptoms similar to lead toxicity, this may also explain the observations by Inuit of strange behaviors on the part of the other crew members later in the expedition.

The study also concluded that significant lead exposure did not occur during the expedition. Until his last few weeks of life, Hartnell’s lead levels were within a healthy, normal range.

“The process of starvation from tuberculosis in Hartnell resulted in the exponential release of previously stored lead from his bones into the blood. Lead concentrations were only high and increasing at the end of his life when he was already likely near death. This explains why previous researchers discovered high lead concentrations in soft tissue; however, they erroneously concluded it was due to recent exposure,” says Dr. Jennie Christensen.

"This is an outstanding application of cutting-edge science, at the interface between archaeology and forensics, to solve a 170-year old mystery. Jennie Christensen and her team are to be congratulated on a great piece of work,” says professor Chris O Hunt, co-editor of Journal of Archaeological Science: Reports which published the study results today.

Funding for this research was provided by Stantec and CLS. Stantec’s Research and Development program, Greenlight supported the funding and development of a method to apply Laser Ablation ICP-MS analysis to determine metals exposure in mammals. 

University of Victoria researcher Jody Spence used the Laser Ablation tool to analyze metal exposure in the samples. University of Saskatchewan (U of S) scientists Joyce McBeth and Nicole Sylvain used synchrotron micro-XRF mapping at the Canadian Light Source to assess environmental contamination in the nail samples. Hing Man (Laurie) Chan used stable isotope analysis at the University of Ottawa to assess sources of protein in Hartnell’s diet.

“Our data with the synchrotron enabled us to determine whether the metals in the thumbnail were from Hartnell’s diet or from contamination of the nail tissues from environmental sources such as coal dust on the ship,” says U of S geological sciences researcher Joyce McBeth. “This provided us with the information we needed to validate Jennie’s quantitative laser analyses.”

“This is a great example of what happens at light source facilities - scientists from different disciplines coming together under one roof, doing great science to solve big problems and, sometimes, great mysteries like this one,” says Dr. Rob Lamb, chief executive office, Canadian Light Source.  

“The University of Ottawa is pleased to support Canada Research Chair Dr. Laurie Chan, whose cutting-edge toxicology and environmental health research helped solve one of Canada’s national mysteries,” says Mona Nemer, Vice-President, Research.

The two ships that embarked on Franklin’s expedition, the HMS Erebus and HMS Terror, were discovered in Canada’s North in 2014 and 2016, respectively. The Inuit Heritage Trust and Canadian Museum of History provided John Hartnell’s thumb and toe nails to the research group for study.

The entire Journal of Archaeological Science: Reports article can be found here.

About Stantec
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The Stantec community unites approximately 22,000 employees working in over 400 locations across six continents. Our work—engineering, architecture, interior design, landscape architecture, surveying, environmental sciences, project management, and project economics, from initial project concept and planning through design, construction, and commissioning—begins at the intersection of community, creativity, and client relationships. With a long-term commitment to the people and places we serve, Stantec has the unique ability to connect to projects on a personal level and advance the quality of life in communities across the globe. Stantec trades on the TSX and the NYSE under the symbol STN.

About TrichAnalytics
TrichAnalytics Inc. spearheads a dynamic and credible analysis approach to uncover data within “growing” biological tissues such as hair, fur, nails and feathers. The data is used to study and monitor temporal changes in environmental, human and wildlife health and make decisions using the best possible information.

“What drives us at TrichAnalytics is uncovering the mysteries around the health of our biological world,” says founder and CEO Dr. Jennie Christensen. “We see the possibilities that have been demonstrated with our research into the Franklin Expedition, and we’re excited to apply them to today’s urgent health questions.”

About the Canadian Light Source
Located at the University of Saskatchewan, the Canadian Light Source is a national research facility, one of the largest science projects in our country’s history, and the brightest light in Canada—millions of times brighter than even the sun—used by over 1,000 scientists from around the world every year in ground-breaking health, environmental, materials, and agricultural research. Visit

Media Contacts

Robyn Quinn              
TrichAnalytics Inc. Med
(250) 415-7020

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